脂肪组织提取试剂盒
分类: 最上层
发布时间: 2018-03-20 18:41
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货号: | AF-023 |
供应商: | InventBiotech |
英文名: | MinuteTM Adipose Tissue Fractionation Kit |
保存条件: | 4℃ |
Application MS analysis, 2D gel analysis, ELISA, SDS-PAGE, immunoblottings, immunoprecipitation, enzyme activity assays, and other applications.
Kit components
1. 15 ml buffer A
2. 15 ml buffer B
3. 1.5 ml microfuge tube (20)
4. Pestles for 1.5 ml tube (2)
5. 20 protein extraction filter cartridges with collection tubes
6. Protein Extraction Powder (2 g)
Storage: Store the kit at 4C
Additional Materials Required: Table-Top Microcentrifuge with a maximum speed of 14,000-16,000 rpm.
Important Product Information The use of protease inhibitors is optional. However if downstream application takes significant amounts of time or the protein extract will be stored for longer period of time, addition of protease inhibitor to buffer A is recommended. For determination of protein concentration BCA assay (Pierce) is recommended. For protein phosphorylation studies, cocktails of phosphatase inhibitors need to be added to buffer A prior to use. Perform
all centrifugation steps in a cold room or in a refrigerated microcentrifuge.
温馨提示:不可用于临床治疗。